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B405 |
PURPLE BROTH BASE |
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Formula |
B405 |
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Ingredients |
gms/lit. |
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Proteose peptone Beef extract Sodium chloride Bromo cresol purple |
10.00 1.00 5.00 0.02 |
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Final pH (at 25°C) : 6.8 + 0.2 |
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Directions |
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Suspend 16 gms. of B405 in 1000ml. distilled water. Add 5-10 gms. of the carbohydrate to be tested. Dispense in tubes as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Alternatively sterilize the basal medium using 900 ml. distilled water and add 100 ml. separately sterilized 5- 10% solution of the desired carbohydrate to it. |
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