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B404 |
ANAEROBIC TRYPTONE SOYA AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Yeast extract Sodium chloride L-Cysteine Vitamin K1 Hemin Agar |
15.00 5.00 5.00 5.00 0.40 0.01 0.005 20.00 |
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Final pH (at 25°C) : 7.5 ± 0.2 |
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Directions |
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Suspend 50.4 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense and sterilize by autoclaving at 15 lbs pressure (121C) for 15 minutes. Cool and pour into plates or tubes as desired. It is recommended that medium be reduced by keeping in anaerobic jar-incubator for 24 hours before use. |
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