B386 

RAPPAPORT VASSILIADIS MEDIUM

Formula

Ingredients 

gms/lit

Papaic digest of soyabean meal 

Sodium chloride

Monopotassium phosphate

Magnesium chloride 

Malachite green 

15.00

 7.20

1.44

36.00

0.036

Final pH (at 25°C) : 5.2 + 0.2

Directions

Suspend 49.2 gms. in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Dispense as desired into tubes & sterilize by autoclaving at 10 lbs pressure (115°C) for15 minutes.

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