B383 

RHIZOBIUM MEDIUM

Formula

Ingredients 

gms/lit.

Mannitol

Dipotassium phosphate

Magnesium sulphate 

Yeast extract 

Agar 

10.00

 0.5

0.2

1.0

20.0

Final pH (at 25°C) : 8.8 + 0.2

Directions 

Suspend 31.8gms. in 1000 ml distilled water. Heat if necessary to dissolve the medium completely.Sterilize by autoclaving at 15 lbs pressure (121°C) for15 minutes.

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