B374 

S.F.P. AGAR BASE

Formula

Ingredients 

gms/lit.

Tryptose

Papaic digest of soyabean meal 

Yeast extract 

Sodium bisulphite 

Ferric ammonium citrate 

Agar 

15.00

5.00

5.00

1.00

1.00

20.00

Final pH (at 25°C) : 7.6+ 0.2

Directions 

Suspend 23.5 gms. in 475ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C. Add 25 ml. of Egg Yolk Emulsion (BF003) and reconstituted contents of 1 vial of S.F.P. Supplement (BF090).Mix well before pouring into sterile petri plates.

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