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B371 |
SPS AGAR MODIFIED |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Ferric citrate Sodium sulphite Sodium thioglycollate Polysorbate 80 Sulphadiazine Polymixin B sulphate Agar |
15.00 10.00 0.50 0.50 0.10 0.05 0.12 0.01 15.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 41.28 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and pour in sterile petri plates containing inoculum. Allow to solidify and if desired, pour the cover layer using about 5 ml. sterile medium. Incubate anaerobically. |
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