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B361 |
XLD AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Yeast extract L-Lysine Lactose Sucrose Xylose Sodium chloride Sodium deoxycholate Sodium thiosulphate Ferric ammonium citrate Phenol red Agar |
3.00 5.00 7.50 7.50 3.50 5.00 2.50 6.80 0.80 0.08 15.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 56.68 gms. in 1000 ml. distilled water. Heat with frequent agitation until the medium Boils. DO NOT AUTOCLAVE OR OVERHEAT. Transfer immediately to a water bath at 50°C. After Cooling, pour into sterile petri plates. It is advisable not to prepare large volumes which will require prolonged heating. |
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