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B360 |
XYLOSE LYSINE AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Xylose L-Lysine Lactose Sucrose Sodium chloride Yeast extract Phenol red Agar |
3.5 5.0 7.5 7.5 5.0 3.0 0.08 13.5 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 45 gms. in 1000 ml. distilled water. Heat with frequent agitation to dissolve completely. Sterilize by autoclaving at (118°C) for 10 minutes. Cool to 50°C to 55°C and add 20 ml sterile solution containing 34% Sodium thiosulphate & 4% Ferric ammonium citrate. Mix well and pour into plates. |
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