B338

TRYPTONE AGAR BASE 

Formula 

B338 

Ingredients 

gms/lit. 

Casein enzymic hydrolysate

Phenol red

Agar

20.00

0.02

3.50

Final pH (at 25°C)

7.4 + 0.2 

Directions 

Suspend 23.52 gms. of B338 in 1000ml. distilled water. Heat to dissolve the medium completely. If desired add required amount of carbohydrate (0.5%) to B338. Dispense in tubes and sterilize by autoclaving at 12 lbs pressure (118 °C) for 15 minutes. Cool the tubed medium in an upright position.

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