|
B329 |
TPEY AGAR BASE |
|
|
Formula |
||
|
Ingredients |
gms/lit. |
|
|
Casein enzymic hydrolysate Yeast extract D-mannitol Sodium chloride Lithium chloride Agar |
10.00 5.00 5.00 20.00 2.00 18.00 |
|
|
Final pH (at 25°C) : 7.2 + 0.2 |
||
|
Directions |
||
|
Suspend 60 gms. in 900ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 12 lbs pressure (118°C) for 15 minutes. Cool to 50°C. Aseptically add 10 ml. of sterile 1% Potassium Tellurite (BF010), 100 ml. Egg Yolk emulsion (BF003) and Polymixin B to a final concentration of 4 mg/l. Mix well and pour into sterile petri plates. |
||