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B310 |
SS AGAR (Salmonella Shigella agar) |
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Formula |
B310 |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Beef extract Lactose Bile salt mixture Sodium citrate Sodium thiosulphate Ferric citrate Brilliant green Neutral red Agar |
5.00 5.00 10.00 8.50 10.00 8.50 1.00 0.00033 0.025 15.00 |
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Final pH (at 25°C) : |
7.0 + 0.2 |
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Directions : |
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Suspend 63 gms. of B310 in 1000 ml. distilled water. Boil with frequent agitation to dissolve the medium completely. DO NOT AUTOCLAVE OR OVERHEATING. Over heating may destroy selectivity of the medium. Cool it about 50°C. Mix & pour into sterile petri plates. |
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