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B308 |
SALT POLYMYXIN BROTH BASE |
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Formula |
B308 |
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Ingredients |
gms/lit. | ||
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Peptic digest of animal tissue Yeast extract Sodium chloride |
9.00 2.70 18.00 |
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Final pH (at 25°C) |
7.4 + 0.2 | ||
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Directions |
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Suspend 14.85 gms. of B308 in 500ml. distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°Cand aseptically add rehydrated contents of 1 vial of Polymyxin B Selective Supplement (BF005). Mix well and dispense as desired. |
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