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B307 |
ROSE BENGAL AGAR BASE |
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Formula |
B307 |
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Ingredients |
gms/lit. |
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Papaic digest of soyabean meal Dextrose Monopotassium phosphate Magnesium sulphate Rose horou Agar |
5.00 10.00 1.00 0.50 0.05 15.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 31.55 gms. of B307 in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45°C & add 2 ml of rehydrated Chloramphenicol selective supplement for each 500 ml of B307. Mix horoughly & pour into sterile petri plate. |
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