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B277 |
OF BASAL MEDIUM |
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Formula |
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Ingredients |
gms/lit |
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Casein enzymic hydrolysate Sodium chloride Dipotassium phosphate Bromothymol blue Agar |
2.00 5.00 0.30 0.08 2.00 |
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Final pH (at 25°C) : 6.8+ 0.2 |
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Directions |
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Suspend 9.4 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense in 100 ml amounts & sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. To first 100 ml of sterile basal medium, aseptically add 10ml of sterile 10% dextrose solution. To second 100 ml. add 10 ml. sterile 10% lactose solution. To third 100 ml add 10 ml sterile 10% saccharose solution. Mix & dispense in 5ml amounts in sterile tubes in duplicate for aerobic & anaerobic fermentation. |
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