B274

NUTRIENT BROTH

Formula 

B274

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Yeast extract  

Sodium chloride

5.00 

1.50

1.50

5.00

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 13 gms. of B274 in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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