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B271 |
SCHAEDLER BROTH |
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Formula |
B271 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Proteose peptone Papaic digest of soyabean meal Yeast Extract Dextrose Sodium chloride Dipotassium hydrogen phosphate Tris hydroxymethyl aminomethane L-Cystine Hemin Agar |
5.67 5.00 1.00 5.00 5.83 1.67 0.83 3.00 0.40 0.01 15.00 |
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Final pH (at 25°C) : 7.6 + 0.2 |
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Directions |
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Suspend 28.41 gms. of B271 in 1000 ml. distilled water. Boil with frequent agitation to dissolve the medium completely. Add 0.02-0.2% agar in broth if desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool and add 5% sterile defibrinated blood if desired. Mix well before dispensing. Avoid overheating and photooxidation of the medium, as it will retard the growth of bacteria. |
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