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B253 |
MCCLUNG TOABE AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Proteose peptone Dextrose Disodium hydrogen phosphate Monopotassium phosphate Sodium chloride Magnesium sulphate Agar |
40.00 2.00 5.00 1.00 2.00 0.10 25.00 |
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Final pH (at 25°C) : 7.6 + 0.2 |
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Directions |
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Suspend 75 gms. in 900ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 20 minutes. Cool to 50°C and aseptically add 100ml of sterile Egg yolk emulsion. Mix well and pour into sterile petri plates. |
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