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B238 |
MacConkey Agar |
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Formula |
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Ingredients |
gm/lit |
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Peptic digest of animal tissue Casein enzymic hydrolysate Pancreatic digest of gelatin Lactose Bile salts Sodium chloride Crystal violet Neutral red Agar |
1.50 1.50 17.00 10.00 1.50 5.00 0.001 0.03 15.00 |
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Final pH (at 25°C): 7.1+ 0.2 |
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Directions |
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Suspend 51.5 gm, in 1000 ml. distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45 – 50°C and pour into sterile petri plates. The surface of the medium should be dry when inoculated. |
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