|
B237 |
MacConkey Agar |
|
|
Formula |
||
|
Ingredients |
gm/lit |
|
|
Peptic digest of animal tissue Lactose Bile salts Neutral red Agar |
20.00 10.00 5.00 0.075 12.00 |
|
|
Final pH (at 25°C): 7.4 + 0.2 |
||
|
Directions |
||
|
Suspend 47.00 gm, in 1000 ml. distilled water. Heat to boiling with gentle swirling to dissolve the agar completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Avoid overheating. Cool to 45 – 50°C and pour into sterile petri plates. The surface of the medium should be dry when inoculated. |
||