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B230 |
LOWENSTEIN JENSEN MEDIUM BASE ( L.J.MEDIUM BASE ) |
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Formula |
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Ingredients |
gms/lit. |
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L-asparagine Monopotassium phosphate Magnesium sulphate Magnesium citrate Potato flour Malachite green |
3.60 2.40 0.24 0.60 30.00 0.40 |
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Final pH (at 25°C) : Self |
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Directions |
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Suspend 37.24 gms. in 600 ml. distilled water containing 12 ml glycerol.(For bovine bacteria or other glycerophobic organisms addition of glycerol is not desirable). Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes . Meanwhile prepare 100 ml whole egg emulsion collected aseptically .Admix egg and base and Gruft Mycobacterial supplement (BF076) (if desired) gently to obtain uniform mixture. Distribute in sterile screw capped tubes .Arrange tubes in slanted position .Coagulate and inspissate the medium in inspissator water bath or autoclave at 85 °C for 45 minutes . |
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