B224

LETHEEN BROTH (AOAC)

Formula 

 B224

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract 

Polysorbate 80 

Sodium chloride 

Lecithin 

10.00

5.00

5.00

5.00

 0.70

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 25.7 gms. of B224 in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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