B207

INDOLE NITRATE MEDIUM (TRYPTONE NITRATE MEDIUM)

Formula

Ingredients

gms/lit

Casein enzymic hydrolysate

Disodium phosphate

Dextrose 

Potassium nitrate 

Agar 

20.00

2.00

1.00

1.00

1.00

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 25 gms in 1000 ml distilled water. Boil to dissolve the medium completely. Dispense in test tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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