B202 

HEART INFUSION BROTH

Formula 

B202

Ingredients 

gms/lit.

Beef heart, infusion from

Tryptose

Sodium chloride

500.00

10.00

5.00 

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 25 gms. of B202 in 1000 ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If desired 5% sterile defibrinated blood may be added. Mix well and pour into sterile petri plates or tubes.

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