B162 

DEOXYCHOLATE AGAR

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Lactose 

Sodium deoxycholate 

Sodium chloride 

Dipotassium phosphate 

Ferric citrate 

Sodium citrate 

Neutral red 

Agar 

10.00

10.00

1.00

5.00

2.00

1.00

1.00

0.03

15.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 45 gms. in 1000 ml. of distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Avoid excessive or prolonged heating during reconstitution.

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