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B158 |
D NASE TEST AGAR BASE |
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Formula |
B158 |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Papaic digest of soyabean meal Deoxyribonucleic acid (DNA) Sodium chloride Agar |
15.00 5.00 2.00 5.00 15.00 |
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Final pH (at 25 C) : 7.3 + 0.2 |
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Directions |
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Suspend 42 gms. of B158 in 1000 ml. distilled water. Heat with frequent agitation to dissolve the medium completely. Sterilize by autoclaving at 12 to 15 lbs pressure (118°C to 121°C) for 15 minutes. Cool to 45°C and pour into sterile petriplates. Add 0.1 gm. Toluidine Blue (BF045) to (B158) before sterilizing the medium or flood the plates with 0.1% Toluidine Blue after incubation as desired |
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