B148 

COLUMBIA BLOOD AGAR BASE (W/1% AGAR)

Formula

Ingredients 

gms/lit.

Peptone, special 

Corn starch 

Sodium chloride 

Agar 

23.00

1.00

5.00

10.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 39 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C before adding heat sensitive compounds.

Print This Page

Close Window