B138 

CETRIMIDE AGAR BASE

Formula

Ingredients 

gms/lit.

Pancreatic Digest of Gelatin

Magnesium Chloride 

Potassium Sulphate 

Cetrimide

Agar 

20.0

1.4

10.0

 0.3

13.60

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 45.30 grams in 1000 ml distilled water. Boil to dissolve the medium completely. Add 10 ml of glycerol heat with frequent agitation & boil for 1 minute. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes.

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