B137 

SULPHUR MEDIUM (Twin Pack)

Formula

Ingredients 

gms/lit.

Part A 

Potassium dihydrogen phosphate

Magnesium sulphate

Ammonium sulphate

Calcium chloride

Ferric chloride

Part B

sulphur, elemental

 

3.00

0.50

0.30

0.25

0.02

 

10.00

Final pH (at 25°C) : 4.8 + 0.2

Directions 

Suspend 4.07 gms. of Part A in 1000ml. distilled water. Dissolve and dispense in 100 ml. amounts in 250 ml. conical flasks. Add 1 gm. of Part B each 100 ml. medium. Sterilize with intermittent steam for 30 minutes on 3 consecutive days.

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