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B131 |
BUSHNELL HASS AGAR |
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Formula |
B131 |
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Ingredients |
gms/lit. |
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Magnesium sulphate Calcium chloride Monopotassium phosphate Dipotassium phosphate Ammonium nitrate Ferric chloride Agar |
0.20 0.02 1.00 1.00 1.00 0.05 20.00 |
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Final pH (at 25°C) : 7.0 + 0.2 |
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Directions |
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Suspend 23.27 gms. of B131 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. A white precipitate prior to sterilization becoming yellow to orange after sterilization is normal. Agar is the solidifying agent. |
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