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B122 |
BREWER THIOGLYCOLLATE MEDIUM |
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Formula |
B122 |
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Ingredients |
gms/lit. |
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Proteose peptone Beef, infusion from Dextrose Sodium chloride Dipotassium phosphate Sodium thioglycollate Methylene blue Agar |
10.00 500.00 5.00 5.00 2.00 0.50 0.002 0.50 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 40.5 gms. of B122 in 1000 ml. distilled water. Boil to dissolve the medium completely. Dispense in tubes or in suitable containers as desired and sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. |
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