B1173 

EE BROTH MOSSEL (ENRICHMENT BROTH MEDIUM E)

Formula

Ingredients 

gms/lit.

Pancreatic digest of gelatin

D-Glucose monohydrate 

Dehydrated Ox-bile 

Disodium hydrogen orthoPhosphate 

Potassium dihydrogen phosphate 

Brilliant green 

10.00

5.00

20.00

8.00

3.00

0.02

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 46.01 gms. in 1000 ml distilled water. Dispense 100 ml amounts in 250 ml flasks.Stopper with cotton plugs or loose fitting caps. Heat to 100 c. boiling water for 30 minutes. Cool rapidly in running water.DO NOT AUTOCLAVE.

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