B1161 

COLUMBIA BLOOD AGAR

Formula

Ingredients 

gms/lit.

Peptone, special 

Corn starch 

Sodium chloride 

Agar 

23.00

1.00

5.00

15.00

Final pH (at 25°C) : 7.3 + 0.2

Directions 

Suspend 44 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes Cool to 45-50°C before adding heat sensitive compounds.

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