|
B116 |
BLOOD AGAR BASE NO.2 |
|
|
Formula |
||
|
Ingredients |
gms/lit. |
|
|
Proteose peptone Liver extract Yeast extract Sodium chloride Agar |
15.00 2.50 5.00 5.00 15.00 |
|
|
Final pH (at 25°C) : 7.4 + 0.2 |
||
|
Directions |
||
|
Suspend 21.25 gms. in 500 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121° C) for 15 minutes. Cool to 40 – 50° C and aseptically add 7% v/v sterile defribinated blood. |
||