B1159 

CAMPYLO THIOGLYCOLLATE MEDIUM

Formula

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Sodium chloride 

Dipotassium phosphate 

Sodium thioglycollate 

L-Cystine 

Sodium sulphite 

Agar 

20.00

2.50

1.50

0.60

0.40

0.20

1.60

Final pH (at 25°C) : 7.0 + 0.2

Directions 

Suspend 26.8 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15lbs pressure (121°C) for 15 minutes. To make the medium selective for Campylobacter species, add reconstituted contents of 2 vials of Campylobacter supplement I. Mix well before dispensing.

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