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B1100 |
M-FC BROTH BASE |
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Formula |
B1100 |
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Ingredients |
gms/lit. |
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Tryptose Proteose peptone Yeast extract Lactose Bile salts mixture Sodium chloride Aniline blue |
10.00 5.00 3.00 12.5 1.50 5.00 0.1 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 37.1 gms. of B1100 in 1000 ml. distilled water containing 10 ml 1% Rosolic Acid. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45°C and add 2 ml of M-FC broth on sterile absorbent pad placed in a sterile petri plates. |
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