B108 

B.A.G.G. BROTH BASE

Formula

Ingredients 

gms/lit.

Tryptose 

Dextrose 

Dipotassium phosphate 

Monopotassium phosphate 

Sodium chloride 

Sodium azide

Bromo cresol purple 

20.00

5.00

4.00

1.50

5.00

 0.50

0.015

Final pH (at 25°C) : 6.9 + 0.2

Directions 

Suspend 36 gms. in 1000 ml. distilled water containing 5 ml glycerol. Dissolve the medium completely and dispense in test tubes in 10 ml amounts. Sterilize by autoclaving at 10 lbs pressure (115°C) for 15 minutes.

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