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B1077 |
KF STREPTOCOCCAL AGAR |
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Formula |
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Ingredients |
gms/lit. |
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Peptone ,special Yeast extract Sodium chloride Sodium glycerophosphate Maltose Lactose Sodium azide Agar |
10.00 10.00 5.00 10.00 20.00 1.00 0.40 20.00 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 76.4 gms. in 1000ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 10 minutes. Cool to 50°C & aseptically add 10 ml. of 1% Triphenyl Tetrazolium chloride (TTC) to sterile medium. Mix well before pouring into sterile petri plates |
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