B107 

AZIDE DEXTROSE BROTH 

Formula 

Ingredients 

gms/lit. 

Peptone, special 

Beef extract

Dextrose 

Sodium chloride 

Sodium azide 

15.00

4.50

7.50

7.50

0.20 

Final pH (at 25°C) : 7.2 + 0.2 

Directions

Suspend 34.7 gms. in 1000 ml. distilled water for preparing single strength broth or use 69.4 gms. in 1000 ml. distilled water for double strength broth. Heat to boiling to ensure complete solution. Dispense in test tubes & sterilize by autoclaving at 12 lbs pressure (118° C) for 15 minutes.

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