B1052 

GASSNER LACTOSE AGAR

Formula

Ingredients 

gms/lit.

Meat peptone

Sodium chloride 

Lactose 

Metachrome yellow 

Water blue 

Agar 

7.00

5.00

50.00

1.25

0.625

13.00

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 76.87 gms in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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