B105

ANAEROBIC AGAR (BREWER) 

Formula 

Ingredients

gms/lit. 

Proteose peptone

Casein enzymic hydrolysate

Yeast extract

Dextrose

Sodium chloride

Sodium thioglycollate

Sodium formaldehyde sulphoxylate

Resazurin

Agar

10.00

5.00

5.00

10.00

5.00

2.00

1.00

0.002

15.00 

Final pH (at 25°C) : 7.2 + 0.2 

Directions

Suspend 53 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min.

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