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B1046 |
FUNGI KIMMIG AGAR BASE |
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Formula |
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Ingredients |
gms/lit. |
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Peptic digest of animal tissue Casein acid hydrolysate Sodium chloride Dextrose Agar |
9.30 4.30 11.40 10.00 15.00 |
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Final pH (at 25°C) : 6.5 + 0.2 |
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Directions |
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Suspend 50 gms. in 1000 ml. distilled water containing 5 ml glycerol. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs (121°C) for 15 minutes. If desired, selective medium is obtained by aseptically adding filtered solution of 0.4 gm. Cycloheximide solution, 40,000 IU Penicillin, 40 mcg Streptomycin, 80 mg Colistin & 100 mg Novobiocin in a previously cooled sterile medium. Mix well & pour in sterile plates. |
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