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B1036 |
FERMENTATION MEDIUM BASE FOR C. PERFRINGENS |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Peptone, special Sodium thioglycollate Agar |
10.00 10.00 0.25 2.00 |
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Final pH (at 25°C) : 7.4 + 0.2 |
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Directions |
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Suspend 22.25 gms. in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Dispense 9 ml of amounts in test tubes containing inverted Durham tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just before use, heat in boiling water or free flowing steam for 10 minute to remove dissolved oxygen and add 1 ml of 1% sterile salicin and raffinose solutions in separate tubes. |
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