B1035

FEELEY GORMAN BROTH (F.G. BROTH)

Formula 

B1035

Ingredients 

gms/lit.

Casein acid hydrolysate

Beef extract 

Starch 

L-cysteine hydrochloride 

Ferric pyrophosphate, soluble

17.50

3.00

1.50

0.40

0.25

Final pH (at 25°C) : 6.9 + 0.2

Directions 

Suspend 23 gms. of B1035 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool 45-50°C and aseptically add rehydrated contents of Legionella Supplement (BF026). Mix well before pouring into sterile tubes or petriplates.

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