B1005

DEY-ENGLEY NEUTRALIZING AGAR

Formula 

B1005 

Ingredients 

gms/lit.

Casein enzymic hydrolysate

Yeast extract 

Dextrose 

Sodium thiosulphate 

Sodium thioglycollate 

Sodium bisulphite 

Lecithin 

Polysorbate 80 

Bromo cresol purple 

Agar 

5.00 

2.50 

10.00 

6.00 

1.00 

2.50 

7.00 

5.00 

0.02 

15.00

Final pH (at 25°C) : 7.6 + 0.2

Directions 

Suspend 54 gms. of B1005 in 1000 ml. distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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