B1004

TRYPTONE BROTH, MODIFIED

Formula 

B1004

Ingredients 

gms/lit. 

Casein enzymic hydrolysate 

Dextrose 

Dipotassium phosphate

Yeast extract 

Bromo cresol purple

10.00

5.00

1.25

1.00

0.04

Final pH (at 25°C) : 6.7 + 0.2

Directions 

Suspend 17.3 gms. of B1004 in 1000 ml. distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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