B1003 

D.C.L.S. AGAR, HAJNA

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Casein enzymic hydrolysate 

Yeast extract 

Beef extract 

Sucrose 

Lactose 

Sodium citrate 

Sodium thiosulphate 

Sodium chloride 

Sodium deoxycholate 

Bromo cresol purple 

Agar 

5.00

5.00

3.00

3.00

7.50

7.50

10.00

5.00

5.00

2.50

0.02

20.00

Final pH (at 25°C) : 7.2 + 0.2

Directions 

Suspend 73.52 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour into sterile petri plates.

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