B097

LISTERIA ENRICHMENT SELECTIVE AGAR 

(TWIN PACK)

Formula 

B097

Ingredients 

gms/lit. 

Part A :

Casein enzymic hydrolysate 

Peptic digest of animal tissue 

Dextrose 

Sodium chloride 

Thiaminium dichloride 

Acriflavin hydrochloride-(Trypaflavin) 

Nalidixic acid 

Agar 

Part B :

Potassium thiocyanate 

 

10.00

10.00

1.00

5.00

0.005

0.01

0.04

13.00

 

37.5

Final pH (at 25°C) : 7.4 + 0.2

Directions 

Suspend 39 gms. of Part A (B097) and 37.5gms of Part B in 1000 ml. distilled water. Heat boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

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