B089 

TMAO MEDIUM (Trimethylamine-N-Oxide Medium)

Formula

Ingredients 

gms/lit.

Peptic digest of animal tissue

Beef extract

Sodium chloride

Yeast extract

Trimethylamine-N-Oxide

Agar

10.00

10.00

5.00

1.00

1.00

2.00

Final pH (at 25°C) : 7.5 + 0.2

Directions 

Suspend 29 gms. in 1000ml. distilled water. Heat to dissolve the medium completely. Dispense 4 ml. in 13 X 100 mm. Screw cap tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool the tubes in an upright position.

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