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B087 |
TARTOFF-HOBBS BROTH (Terrific Broth) |
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Formula |
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Ingredients |
gms/lit. |
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Casein enzymic hydrolysate Yeast extract Monopotassium phosphate Dipotassium phosphate |
12.00 24.00 9.40 2.20 |
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Final pH (at 25°C) : 7.2 + 0.2 |
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Directions |
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Suspend 47.6 gms. in 1000ml. distilled water. Heat to boiling to dissolve the medium completely. Add 4 ml. of glycerol to the medium. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. |
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