B042

TCBS AGAR / (SELECTIVE)

Formula 

B042 

Ingredients 

gms/lit. 

Peptone special

Proteose peptone 

Yeast extract 

Sodium thiosulphate 

Sodium citrate 

Sodium cholate

Oxgall 

Sucrose 

Sodium chloride 

Ferric citrate 

Bromo thymol blue 

Thymol blue 

Agar 

--- 

10.00

5.00 

10.00 

10.00 

 --- 

8.00 

20.00

10.00

1.00 

0.04 

0.04 

15.00 

Final pH (at 25°C)

8.6 + 0.2 

Directions 

Suspend 89 gms. in 1000 ml. distilled water. Boil to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 50°C and pour into petri plates.

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